This system offers super-resolution microscopy that provides the ability to resolve small structures down to 20nm using SMLM technology. It’s perfectly situated for small particle and extracellular vesicle characterization. The small size of the device makes it an ideal instrument for use in a BSL-3 environment.
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- dSTORM – Stochastic Optical Reconstruction Microscopy
- PAINT – Point Accumulation for Imaging in Nanoscale Topography
- PALM – PhotoActivated Light Microscopy
- SPT – Single Particle Tracking
- Imaging technology
- 2D and 3D SMLM
- 3D SPT
- TRIF: Total internal reflection fluorescence
- FRET: Forster resonance energy transfer
- Spatiotemporal resolution
- Up to 20nm in XY, 50nm in Z via SMLM
- Up to 10ms/100Hz with SPT, FRET, TRIF
- Imaging channels
- 4 interlaced, 2 simultaneous
- <10nm channel mapping resolution
- Field of view
- 50um X 80um with a 100x objective
- 250um X 400um with a 20x objective
- Illumination
- 405nm, 488nm, 561nm, 649nm with up to 1W
- Up to 10kW/cm2 in the sample plane
- Flat-field homogenous laser illumination
- Continuous illumination angle from epi to HILO to TIRF
- Light patterning
- Digital micromirror device-based light patterning
- Objectives
- Numerical aperture (NA) from 0.45 to 1.50
- Magnification from 20x to 100x